Figure S2.
Cdk5Rap2 and γ-tubulin abundance scales with spindle size. (A) Immunofluorescence images of 2:2, 1:1, 1:0, and 0:0 RPE1 Centrin1-GFP metaphase cells stained with DAPI (blue), GFP-centrin1 (green), and Cdk5Rap2 antibody (magenta). Scale bars = 10 μm. Correlation plots between the relative Cdk5Rap2 distribution (x axis) and the SAI (y axis) for 2:2, 1:1, 1:0, and 0:0 cells. Dots represent single-cell values. The light green line indicates the slope of the linear regression. For each plot, the Pearson correlation coefficient, its associated P value, the number of cells analyzed, and the slope of the linear regression are indicated. (B) Immunofluorescence images of 2:2, 1:1, 1:0, and 0:0 RPE1 GFP-centrin1 metaphase cells stained with DAPI (blue), GFP-centrin1 (green), and γ-tubulin antibody (magenta). Note that these images are also shown in Fig. 1 A Scale bars = 10 μm. Correlation plots between the relative γ-tubulin distribution (x axis) and the SAI (y axis) for 2:2, 1:1, 1:0, and 0:0 cells. Dots represent single-cell values. The light green line indicates the slope of the linear regression. For each plot, the Pearson correlation coefficient, its associated P value, the number of cells analyzed, and the slope of the linear regression are indicated.

Cdk5Rap2 and γ-tubulin abundance scales with spindle size. (A) Immunofluorescence images of 2:2, 1:1, 1:0, and 0:0 RPE1 Centrin1-GFP metaphase cells stained with DAPI (blue), GFP-centrin1 (green), and Cdk5Rap2 antibody (magenta). Scale bars = 10 μm. Correlation plots between the relative Cdk5Rap2 distribution (x axis) and the SAI (y axis) for 2:2, 1:1, 1:0, and 0:0 cells. Dots represent single-cell values. The light green line indicates the slope of the linear regression. For each plot, the Pearson correlation coefficient, its associated P value, the number of cells analyzed, and the slope of the linear regression are indicated. (B) Immunofluorescence images of 2:2, 1:1, 1:0, and 0:0 RPE1 GFP-centrin1 metaphase cells stained with DAPI (blue), GFP-centrin1 (green), and γ-tubulin antibody (magenta). Note that these images are also shown in Fig. 1 A Scale bars = 10 μm. Correlation plots between the relative γ-tubulin distribution (x axis) and the SAI (y axis) for 2:2, 1:1, 1:0, and 0:0 cells. Dots represent single-cell values. The light green line indicates the slope of the linear regression. For each plot, the Pearson correlation coefficient, its associated P value, the number of cells analyzed, and the slope of the linear regression are indicated.

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