Figure 3.
The spindle pole abundance of proteins regulating microtubule nucleation scales with half-spindle size. (A) Immunofluorescence images of 2:2, 1:1, 1:0, and 0:0 RPE1 GFP-centrin1 metaphase cells stained with DAPI (blue), GFP-centrin1 (green), and pericentrin antibody (magenta). Scale bars = 10 μm. (B) Correlation plots between the relative pericentrin distribution (x axis) and the SAI (y axis) for 2:2, 1:1, 1:0, and 0:0 cells. Dots represent single-cell values. The light green line indicates the slope of the linear regression. For each plot, the Pearson correlation coefficient, its associated P value, the number of cells analyzed, and the slope of the linear regression are indicated. The data for all the proteins can be found in Table 1.

The spindle pole abundance of proteins regulating microtubule nucleation scales with half-spindle size. (A) Immunofluorescence images of 2:2, 1:1, 1:0, and 0:0 RPE1 GFP-centrin1 metaphase cells stained with DAPI (blue), GFP-centrin1 (green), and pericentrin antibody (magenta). Scale bars = 10 μm. (B) Correlation plots between the relative pericentrin distribution (x axis) and the SAI (y axis) for 2:2, 1:1, 1:0, and 0:0 cells. Dots represent single-cell values. The light green line indicates the slope of the linear regression. For each plot, the Pearson correlation coefficient, its associated P value, the number of cells analyzed, and the slope of the linear regression are indicated. The data for all the proteins can be found in Table 1.

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