Diffusion dynamics of autophagy factor foci. (A) Step size distributions of LC3B-positive and -negative ULK1-Halo, and Halo-ATG2A trajectories fit with a two-state diffusion model (black line) encompassing tethered (red) and untethered (blue) populations in the different media conditions. (B) Diffusion coefficients of the tethered and untethered populations of LC3B-positive and -negative ULK1-Halo, and Halo-ATG2A trajectories in the different media conditions, derived from the fits shown in Fig. S6 A. Statistical significance was assessed using a two-tailed t-test (*P < 0.05).