Contribution of the ULK1 complex to starvation-induced autophagy protein foci formation. (A) Formation rate of total, LC3B-positive, and LC3B-negative Halo-ATG13 foci in control, ULK1, FIP200, and ATG101 knock-out cells in various media conditions. (B) Lifetimes of LCB3-positive and LC3B-negative Halo-ATG13 foci in control, ULK1, FIP200, and ATG101 knock-out cells in various media conditions. Statistical significance was assessed by one-way ANOVA. The letter assignments denote statistically significant differences among groups at P = 0.05, as determined by a Bonferroni post-hoc test.