Figure S4. Analysis of LC3B G120A... | Rockefeller University Press

Figure S4.

$Analysis of LC3B G120A aggregates, colocalization of Halo-ATG13 with GFP-P62, and assessment of alternate data processing. (A) K-FOCUS analysis of Halo-ATG13 colocalization with GFP-LC3B G120A expressed by viral transduction in cells grown in complete media, media lacking amino acids and FBS, or media lacking glucose. (B) K-FOCUS analysis of Halo-ATG13 colocalization with stably expressed GFP-P62 in cells grown in complete media, media lacking amino acids and FBS, or media lacking glucose. (C) Quantification of colocalization kinetics of single cells in Fig. 3 A and Fig. S1 using K-FOCUS including total, colocalized, and non-colocalized phagophore formation rates. Data was binned 2 × 2 to a pixel size of 217 nm. (D) Quantified fractions of LC3+ foci/cell from imaging in Fig. 3 A and Fig. S1. For B, C, and D, the box indicates the interquartile range, the whiskers the 10–90% confidence interval, the square indicates the average, and the horizontal line is the median. Statistical significance was assessed by one-way ANOVA. The letter assignments denote statistically significant differences among groups at P = 0.05, as determined by a Bonferroni post-hoc test.$

Analysis of LC3B G120A aggregates, colocalization of Halo-ATG13 with GFP-P62, and assessment of alternate data processing. (A) K-FOCUS analysis of Halo-ATG13 colocalization with GFP-LC3B G120A expressed by viral transduction in cells grown in complete media, media lacking amino acids and FBS, or media lacking glucose. (B) K-FOCUS analysis of Halo-ATG13 colocalization with stably expressed GFP-P62 in cells grown in complete media, media lacking amino acids and FBS, or media lacking glucose. (C) Quantification of colocalization kinetics of single cells in Fig. 3 A and Fig. S1 using K-FOCUS including total, colocalized, and non-colocalized phagophore formation rates. Data was binned 2 × 2 to a pixel size of 217 nm. (D) Quantified fractions of LC3+ foci/cell from imaging in Fig. 3 A and Fig. S1. For B, C, and D, the box indicates the interquartile range, the whiskers the 10–90% confidence interval, the square indicates the average, and the horizontal line is the median. Statistical significance was assessed by one-way ANOVA. The letter assignments denote statistically significant differences among groups at P = 0.05, as determined by a Bonferroni post-hoc test.