Figure 3. K-FOCUS analysis of the... | Rockefeller University Press

Figure 3.

$K-FOCUS analysis of the kinetics of autophagy factor foci formation under nutrient starvation. (A) Live-cell images of Halo-WIPI2 and GFP-LC3B in control and starvation medium (scale bar = 5 µm). (B) Quantification of colocalization kinetics of single cells in A and Fig. S1 using K-FOCUS, including total, colocalized, and non-colocalized phagophore formation rates. Data was downsampled to a pixel size of 370 nm. (C) Quantified fractions of LC3+ foci/cell from imaging in A and Fig. S1. For B and C, the box indicates the interquartile range, the whiskers the 10–90% confidence interval, the square indicates the average, and the horizontal line is the median. Statistical significance was assessed by one-way ANOVA. The letter assignments denote statistically significant differences among groups at P = 0.05, as determined by a Bonferroni post-hoc test.$

K-FOCUS analysis of the kinetics of autophagy factor foci formation under nutrient starvation. (A) Live-cell images of Halo-WIPI2 and GFP-LC3B in control and starvation medium (scale bar = 5 µm). (B) Quantification of colocalization kinetics of single cells in A and Fig. S1 using K-FOCUS, including total, colocalized, and non-colocalized phagophore formation rates. Data was downsampled to a pixel size of 370 nm. (C) Quantified fractions of LC3+ foci/cell from imaging in A and Fig. S1. For B and C, the box indicates the interquartile range, the whiskers the 10–90% confidence interval, the square indicates the average, and the horizontal line is the median. Statistical significance was assessed by one-way ANOVA. The letter assignments denote statistically significant differences among groups at P = 0.05, as determined by a Bonferroni post-hoc test.