Septin localization with respect to tyrosinated, detyrosinated, and acetylated microtubules. (A) Maximum intensity projections of super-resolution confocal microscopy images of MDCK cells stained for endogenous SEPT7 and tyrosinated α-tubulin. Arrowheads point to SEPT7 colocalizing with tyrosinated microtubules. Scale bars, 5 μm. (B) Maximum intensity projections of super-resolution confocal microscopy images of MDCK cells stained for endogenous SEPT7 and detyrosinated α-tubulin. The outlined perinuclear regions 1 and 2 (dashed rectangle) are shown in higher magnification, and arrowheads point to SEPT7-coated microtubule segments with low levels of detyrosinated α-tubulin. Scale bars, 5 μm. (C) Maximum intensity projections of super-resolution confocal microscopy images of MDCK cells stained for endogenous SEPT7 and acetylated tubulin. The perinuclear region outlined with a dashed rectangle is shown in higher magnification, and arrowheads point to areas of SEPT7 enrichment with low levels of acetylated tubulin. Scale bars, 5 μm.