The sequence alignment and comparison of the SET domain of Rkm1 and other methyltransferases. (A) The sequence alignment (Jalview Alignment by ClustalW) of Rkm1, SETD6 (human, GenBank AAH22451), LSMT (PDB accession no. 2H2E), and DIM5 (PDB accession no. 1ML9). s: substrate binding; aa: SAM binding; c: catalytic site. (B) The structurally conserved core of SET domain is shown in yellow and green, and the insertion region is shown in gray. The active sites, Y273 in Rkm1 and Y285 in SETD6 (3QXY), are shown in blue. The SAM is shown in atom type.