Figure S1.
Bcp1 mutant did not change the protein level of Rkm1 and vice versa. (A) The growth tests of rkm1Δ at different conditions. (B) The size exclusion chromatography (left). The result of Coomassie blue gels (right). The y-axis is the normalized intensity of UV280 nm. The x-axis is the elution volume of the Superdex 200 column. (C) The growth tests of WT and bcp1ts containing vector, 2μ RKM1, or 2μ RPL23. (D) The protein level of Bcp1 was detected in WT and rkm1Δ. (E) The protein level of Rkm1 was detected under overexpression or depletion of Bcp1. (F) The localization of Bcp1-GFP was visualized in wild-type and GAL::RPL23. Overnight cells were subcultured in a medium containing 2% galactose for 2 h and 2% glucose was added for another 4 h before examination with fluorescence microscopy. To visualize the localization of Rkm1-GFP in wild-type and bcp1ts at log phase were shifted to 37°C for 2 h. Source data are available for this figure: SourceData FS1.

Bcp1 mutant did not change the protein level of Rkm1 and vice versa. (A) The growth tests of rkm1Δ at different conditions. (B) The size exclusion chromatography (left). The result of Coomassie blue gels (right). The y-axis is the normalized intensity of UV280 nm. The x-axis is the elution volume of the Superdex 200 column. (C) The growth tests of WT and bcp1ts containing vector, 2μ RKM1, or 2μ RPL23. (D) The protein level of Bcp1 was detected in WT and rkm1Δ. (E) The protein level of Rkm1 was detected under overexpression or depletion of Bcp1. (F) The localization of Bcp1-GFP was visualized in wild-type and GAL::RPL23. Overnight cells were subcultured in a medium containing 2% galactose for 2 h and 2% glucose was added for another 4 h before examination with fluorescence microscopy. To visualize the localization of Rkm1-GFP in wild-type and bcp1ts at log phase were shifted to 37°C for 2 h. Source data are available for this figure: SourceData FS1.

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