YAP1/TAZ regulates N-cad surface level during PHGG migration. (A) Western blot analysis of control or YAP1/TAZ siRNA-treated cells. Vinculin is shown as a loading control. (B–D) Representative images and quantification of total N-cad in low-density cultures of control and YAP1/TAZ-depleted cells. Maximum intensity projections. Scale bars, 20 μm. a.u., arbitrary units. (E and F) Representative histograms and quantification of N-cad surface levels in low-density cultures of control and YAP1/TAZ-depleted cells measured by flow cytometry. (G and H) Representative images and quantification of surface N-cad ECD antibody binding to migrating leader (L) and follower (F) cells. Scale bars, 10 μm. (I) Model diagram: YAP1 activation regulated by cell density affects N-cad surface expression and stimulates migration in neurons and glia but inhibits migration in ECM. (C and D) Unpaired t test. (F) Two-way ANOVA Šídák’s multiple comparisons test. (H) Two-way ANOVA Tukey’s multiple comparisons test. Error bars show mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. ns, not significant. (C and D)N = 30 cells. (F)N = 5 experiments. (H)N = 4 experiments. Source data are available for this figure: SourceData F8.