Figure S5.
YAP1/TAZ regulates PHGG migration and N-cad endocytosis. (A) Western blot analysis of control, YAP1, TAZ, or YAP1/TAZ siRNAs in PBT-05 cells. Vinculin is shown as a loading control. (B) Cumulative migration distance on laminin for 24 h. Ordinary one-way ANOVA Šídák’s multiple comparisons test. N = 9–16 spheroids, three experiments. Error bars indicate mean ± SEM. (C and D) Representative images and quantification of internalized N-cad Abs at 40 min incubation. N = 4–13 spheroids, three experiments. (E and F) Representative images and quantification of internalized Alexa Fluor 568-conjucated transferrin at 40 min incubation. N = 5–7 spheroids, two experiments. (D and F) Two-way ANOVA Šídák’s multiple comparisons test. *P < 0.05, **P < 0.001, ***P < 0.001, ****P < 0.0001. ns, not significant. Source data are available for this figure: SourceData FS5.

YAP1/TAZ regulates PHGG migration and N-cad endocytosis. (A) Western blot analysis of control, YAP1, TAZ, or YAP1/TAZ siRNAs in PBT-05 cells. Vinculin is shown as a loading control. (B) Cumulative migration distance on laminin for 24 h. Ordinary one-way ANOVA Šídák’s multiple comparisons test. N = 9–16 spheroids, three experiments. Error bars indicate mean ± SEM. (C and D) Representative images and quantification of internalized N-cad Abs at 40 min incubation. N = 4–13 spheroids, three experiments. (E and F) Representative images and quantification of internalized Alexa Fluor 568-conjucated transferrin at 40 min incubation. N = 5–7 spheroids, two experiments. (D and F) Two-way ANOVA Šídák’s multiple comparisons test. *P < 0.05, **P < 0.001, ***P < 0.001, ****P < 0.0001. ns, not significant. Source data are available for this figure: SourceData FS5.

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