YAP1 signaling and wound healing gene expression is increased in leader cells. (A and B) Representative images and quantification of YAP1 localization in leader and follower cells in PBT-24 and PBT-29 DMG cells migrating on Matrigel for 72 h. Scale bars, 20 μm. N = 5 spheroids, three experiments. Two-way ANOVA Šídák’s multiple comparisons test. Error bars indicate ±SEM. **P < 0.01, ***P < 0.001. (C) RNASeq analysis of cadherin, catenin, YAP1, and TAZ expression in leader and follower cells. (D) Gene ontology functional enrichment analysis of differentially expressed genes (DEGs) between leader and follower cells.