Role of intercellular N-cad homotypic interactions and importance of N-cad for catenin localization. (A and B) Representative images of Ncad^WT and Ncad^W161A expressing PBT-05 cells migrating on cerebellar neurons or laminin. (C) Representative images of PBT-05 cells migrating on control or N-cad siRNA-treated mouse astrocytes. Scale bars, 200 μm. (D and E) Images of PBT-05 cells expressing control, p120-catenin (p120-ctn), β-catenin (β-ctn), or α-catenin (α-ctn) shRNAs, migrating on mouse astrocytes or laminin. Scale bars, 200 μm. (F and G) N-cad, p120-catenin β-catenin, and α-catenin protein levels in control and N-cad-depleted cells. Protein expression levels were normalized to β-tubulin as a loading control. Two-way ANOVA uncorrected Fisher’s LSD test. N = 3 Western blots. Error bars show mean ± SEM. *P < 0.05, ****P < 0.0001. (H) Localization of p120-catenin, β-catenin, and α-catenin in control or N-cad-depleted cells. Dashed lines indicate clusters of N-cad-depleted cells. Arrowheads indicate cell-cell junctions and arrows indicate intracellular vesicles. Scale bars, 20 μm. Source data are available for this figure: SourceData FS2.