Conditions that alter GTP availability similarly modulate rates of induced GR import. (A) Import rates of BJ-5ta cells expressing LINuS and treated with importazole (control n = 22, importazole n = 24; two independent replicates). (B) Representative confocal images of pre-photoactivation and 3.5 min post-photoactivation of LINuS in BJ-5ta cells treated with importazole. (C and D) Quantitative measurements of dexamethasone-induced import of GR-GFP in BJ-5ta cells with or without importazole (Control n = 22; importazole n = 25; two independent replicates), with representative confocal images (D) of cells with either no dexamethasone treatment (−Dex) or 15 min after dexamethasone treatment (+Dex). (E and F) Graphs of dexamethasone-induced nuclear import of GR-GFP in BJ-5ta cells (E) plated for either 2 (n = 36) or 24 h (n = 38; two independent replicates) or (F) depleted of SUN2 (siControl n = 105, siSUN2 n = 107; two independent replicates). Significance was calculated using unpaired t test (A, C, E, and F). P***<0.001, P****<0.0001. Scale bars 10 µm. Error bars represent ± SEM.