Validation of siSUN2 specificity. (A and B) Representative fluorescence images of BJ-5ta cells stably expressing LINuS (parental) or co-expressing LINuS and a V5-tagged mouse SUN2 (V5-SUN2^Mm) and transfected with either siControl or siSUN2 to transiently knockdown endogenous SUN2. Cells were subsequently labeled with antibodies against SUN2 (A) or V5 (B) to validate the resistance of exogenous mouse SUN2 to human SUN2 siRNAs. (C and D) Import and export rates of BJ-5ta cells stably expressing LINuS and V5-SUN2^Mm and depleted of endogenous SUN2 (siControl n = 94, siSUN2 n = 91). Results are from two independent replicates. Scale bar 10 µm.