Figure 4.
Disruption of the LINC complex enhances GTP availability and rates of NCT. (A) Quantification of GEVAL ratiometric signal (405 nm/488 nm) in BJ-5ta cells expressing GEVALnull or GEVAL30 and codepleted of SUN1 and SUN2 (SUN1/2), SUN1, or SUN2 (GEVALNull siControl n = 130, siSUN1/2 n = 115, siSUN1 = 173, siSUN2 = 128. GEVAL30 siControl n = 173, siSUN1/2 n = 203, siSUN1 n = 227, siSUN2 n = 153). Results are from three independent replicates. (B) Import and export rates of BJ-5ta cells stably expressing LINuS and codepleted of SUN1 and SUN2 (SUN1/2), SUN1, or SUN2. siControl n = 125, siSUN1/2 n = 47, siSUN1 n = 61, siSUN2 n = 58. Results are from three independent replicates. (C) Import and export rates of MCF10A cells stably expressing LINuS and transiently transfected with ss-GFP-KDEL (n = 53) or ss-SUN1L-GFP-KDEL (n = 46) from three independent replicates. (D) Quantification of GEVAL ratiometric signal (405 nm/488 nm) in BJ-5ta cells expressing GEVALnull or GEVAL30 depleted of Nesprin1, Nesprin2, or Nesprin3 (GEVALNull siControl n = 138, siNesprin1 n = 144, siNesprin2 n = 107, siNesprin3 n = 132; GEVAL30 siControl n = 180, siNesprin1 n = 154, siNesprin2 n = 190, siNesprin3 n = 144). Results are from three independent replicates. (E) Import and export rates of BJ-5ta cells stably expressing LINuS and depleted of Nesprin1, Nesprin2, or Nesprin3 (siControl n = 125, siNesprin1 n = 43, siNesprin2 n = 39, siNesprin3 n = 39; three independent replicates). Significance was calculated using t test (C) or one-way ANOVA with Dunnett’s post hoc (A, B, D, and E). ns P > 0.05, P*<0.05, P**<0.01, P***<0.001, P****<0.0001.

Disruption of the LINC complex enhances GTP availability and rates of NCT. (A) Quantification of GEVAL ratiometric signal (405 nm/488 nm) in BJ-5ta cells expressing GEVALnull or GEVAL30 and codepleted of SUN1 and SUN2 (SUN1/2), SUN1, or SUN2 (GEVALNull siControl n = 130, siSUN1/2 n = 115, siSUN1 = 173, siSUN2 = 128. GEVAL30 siControl n = 173, siSUN1/2 n = 203, siSUN1 n = 227, siSUN2 n = 153). Results are from three independent replicates. (B) Import and export rates of BJ-5ta cells stably expressing LINuS and codepleted of SUN1 and SUN2 (SUN1/2), SUN1, or SUN2. siControl n = 125, siSUN1/2 n = 47, siSUN1 n = 61, siSUN2 n = 58. Results are from three independent replicates. (C) Import and export rates of MCF10A cells stably expressing LINuS and transiently transfected with ss-GFP-KDEL (n = 53) or ss-SUN1L-GFP-KDEL (n = 46) from three independent replicates. (D) Quantification of GEVAL ratiometric signal (405 nm/488 nm) in BJ-5ta cells expressing GEVALnull or GEVAL30 depleted of Nesprin1, Nesprin2, or Nesprin3 (GEVALNull siControl n = 138, siNesprin1 n = 144, siNesprin2 n = 107, siNesprin3 n = 132; GEVAL30 siControl n = 180, siNesprin1 n = 154, siNesprin2 n = 190, siNesprin3 n = 144). Results are from three independent replicates. (E) Import and export rates of BJ-5ta cells stably expressing LINuS and depleted of Nesprin1, Nesprin2, or Nesprin3 (siControl n = 125, siNesprin1 n = 43, siNesprin2 n = 39, siNesprin3 n = 39; three independent replicates). Significance was calculated using t test (C) or one-way ANOVA with Dunnett’s post hoc (A, B, D, and E). ns P > 0.05, P*<0.05, P**<0.01, P***<0.001, P****<0.0001.

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