Figure S10.
Cofilin cluster severing rate on single actin filaments. (A) Cofilin cluster severing rate on single actin filaments from three independent experiments (n = 19, 88, 45 filaments from lighter to darker blue curves). Single exponential fits (black lines) yield cofilin cluster severing rates on single filaments of 1.7, 2.15, and 2.4 × 10−3 s−1. (B) Cofilin cluster severing rate on single actin filaments at 100 and 200 nM cofilin (n = 31 and 53 filaments, respectively). Single exponential fits (black lines) yield cofilin cluster severing rates of 2.86 and 2.31 × 10−3 s−1.
