Figure S7.
Details of ECM fibers. (A) Focused view on the banding pattern of collagen fibers. The shown tomogram was reconstructed using a SIRT-like filter to enhance the visibility of the banding pattern. The typical collagen repeat pattern of a 41 nm wide band followed by a 26 nm narrow band, summing up to an overall repeat of 67 nm, is indicated. (B) Fiber diameter of 79 collagen fibers as measured in cross-section view (to reduce missing-wedge effect-caused inaccuracies). Each single measurement is shown as a dot in a violin plot. A red line indicates the median. On average, collagen fibers have a diameter of ∼38 nm (SD = 6.5 nm), ranging from 26 to 60 nm. The distribution in diameter of the measured fibers is shown in more detail in the pie-chart on the right. (C and D) Characterization of small ECM fibers by measuring their diameter (C) and node-to-node repeat distance (D) in IsoNet-corrected tomograms. (C) Four exemplary images showing one or more small ECM fibers (left). Their diameter has been measured at their widest point. As fibers were not round, the average of two distances, measured perpendicular to each other, was calculated. 110 individual measurements are displayed in the respective violin plot shown on the right, with the red line indicating the median. The average bead diameter is 15.74 nm (SD = ±1.31). (D) The node-to-node distance of the small ECM fibers was determined for 54 individual measurements. Exemplary images of IsoNet-corrected tomogram regions used for measurements are shown on the left. Below, one exemplary filament is traced in yellow, with the tracing shown as standalone in black next to it. The measurements are summarized in the violin plot on the right, the red line indicates the median. Overall, the node distance is at average 60.30 nm (SD = ±7.82 nm). Scale bar dimensions are annotated within the figure.

Details of ECM fibers. (A) Focused view on the banding pattern of collagen fibers. The shown tomogram was reconstructed using a SIRT-like filter to enhance the visibility of the banding pattern. The typical collagen repeat pattern of a 41 nm wide band followed by a 26 nm narrow band, summing up to an overall repeat of 67 nm, is indicated. (B) Fiber diameter of 79 collagen fibers as measured in cross-section view (to reduce missing-wedge effect-caused inaccuracies). Each single measurement is shown as a dot in a violin plot. A red line indicates the median. On average, collagen fibers have a diameter of ∼38 nm (SD = 6.5 nm), ranging from 26 to 60 nm. The distribution in diameter of the measured fibers is shown in more detail in the pie-chart on the right. (C and D) Characterization of small ECM fibers by measuring their diameter (C) and node-to-node repeat distance (D) in IsoNet-corrected tomograms. (C) Four exemplary images showing one or more small ECM fibers (left). Their diameter has been measured at their widest point. As fibers were not round, the average of two distances, measured perpendicular to each other, was calculated. 110 individual measurements are displayed in the respective violin plot shown on the right, with the red line indicating the median. The average bead diameter is 15.74 nm (SD = ±1.31). (D) The node-to-node distance of the small ECM fibers was determined for 54 individual measurements. Exemplary images of IsoNet-corrected tomogram regions used for measurements are shown on the left. Below, one exemplary filament is traced in yellow, with the tracing shown as standalone in black next to it. The measurements are summarized in the violin plot on the right, the red line indicates the median. Overall, the node distance is at average 60.30 nm (SD = ±7.82 nm). Scale bar dimensions are annotated within the figure.

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