Additional examples of segmented cryo-electron tomograms. (A and B) Segmentations of two exemplary IsoNet-processed tomograms acquired on the cryo-lift-out lamella shown in Fig. S4 A. The top panels show a single central slice (thickness of 1.71 nm) of each respective tomogram. Cell and ECM fibers run in an oblique angle to the lamella, resulting in a side view of intra- and extracellular filaments. Middle panels show segmentations of tomograms overlaid over the tomogram slice. The bottom panels show an oblique view of just the segmentation volume. Scale bars indicate 100 nm. The color scheme for the different segmented cellular and ECM components is described in the figure.