Figure S4.
Additional examples of completely vitrified cryo-lift-out lamellae. (A and B) Two additional, completely vitrified lamellae of day 16 CDMs, high-pressure frozen in degassed 0.1 M PB with 10% dextran. White dashed rectangles denote the areas of acquisition for the tomograms shown in Fig. S5. An overview map of each whole lamella is shown in A1 and B1. The CDM height is indicated on the left, starting from 0 µm close to the EM grid and up to 21 and 18 μm at the surface of the CDM, respectively. Areas featuring structures of interest are depicted at higher magnification in A2 and B2. Three ROIs are highlighted in each example and shown at higher magnification on the right (A3–A5 and B3–B5). Cell areas are annotated with a transparent red color, ECM areas are labeled as such. Scale bar dimensions are annotated in the figure. The experimental conditions of the shown CDMs are identical to the data shown in Fig. 2.

Additional examples of completely vitrified cryo-lift-out lamellae. (A and B) Two additional, completely vitrified lamellae of day 16 CDMs, high-pressure frozen in degassed 0.1 M PB with 10% dextran. White dashed rectangles denote the areas of acquisition for the tomograms shown in Fig. S5. An overview map of each whole lamella is shown in A1 and B1. The CDM height is indicated on the left, starting from 0 µm close to the EM grid and up to 21 and 18 μm at the surface of the CDM, respectively. Areas featuring structures of interest are depicted at higher magnification in A2 and B2. Three ROIs are highlighted in each example and shown at higher magnification on the right (A3–A5 and B3–B5). Cell areas are annotated with a transparent red color, ECM areas are labeled as such. Scale bar dimensions are annotated in the figure. The experimental conditions of the shown CDMs are identical to the data shown in Fig. 2.

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