Genotyping results of knock-in lines and example analysis. (A) DNA gel of control cell lines and homozygous knock-in of the Halotag to the DYNC1H1 and DCTN4 locus. (B) DNA gel of control cell lines and homozygous knock-in of the Halotag to the NDEL1 locus. (C) DNA gel of control cell lines and heterozygous knock-in of the Halotag to the PAFAH1B1 (LIS1) locus. Comparison between kymograph and spots tracked with Trackmate. (D) Kymograph from Fig. 3 A, retrograde dynein tracks (Halo-DYNC1H1). (E) Tracks extracted from Trackmate have been overlaid on the original kymograph from Fig. 3 A in green. Source data are available for this figure: SourceData FS3.