Figure 4.
Dynein machinery moves long-range retrogradely along the axon. (A) Schematic of microfluidic device showing treatment in the axonal compartment with JFX halo ligand and imaging in the somatodendritic compartment. Example kymographs of retrograde dynein (Halo-DYNC1H1), dynactin (Halo-ACTR10), LIS1 (PAFAH1B1-Halo), and NDEL1 (Halo-NDEL1) movement in 21–23 DPI neurons. (B) The frequency of retrograde motile events in dynein, dynactin, LIS1, and NDEL1 in 21–23 DPI neurons (dynein: 162 tracks, 21 videos, N = 6; dynactin: 68 tracks, 10 videos, N = 4; LIS1: 38 tracks, 14 videos, N = 6; NDEL1: 24 tracks, 10 videos, N = 3). Dynein versus NDEL1: **P = 0.0088, dynactin versus NDEL1: *P = 0.026, dynein versus dynactin: P = 0.81, LIS1 versus dynactin: P = 0.15, LIS1 versus dynein: P = 0.06, LIS1 versus NDEL1: P = 0.28, Kruskal–Wallis test, Dunn post hoc test. (C) Graph showing the speed of retrograde dynein, dynactin, LIS1, and NDEL1 particles in 21–23 DPI neurons. Dynein versus dynactin: P = 0.22, NDEL1 versus dynactin: P = 0.25, LIS1 versus dynactin: P = 0.07, dynein versus NDEL1: P = 0.90, dynein versus LIS1: P = 0.50, NDEL1 versus LIS1: P = 0.68. Boxplots shows median, first, and third quartiles. Upper/lower whiskers extend to 1.5× the interquartile range.

Dynein machinery moves long-range retrogradely along the axon. (A) Schematic of microfluidic device showing treatment in the axonal compartment with JFX halo ligand and imaging in the somatodendritic compartment. Example kymographs of retrograde dynein (Halo-DYNC1H1), dynactin (Halo-ACTR10), LIS1 (PAFAH1B1-Halo), and NDEL1 (Halo-NDEL1) movement in 21–23 DPI neurons. (B) The frequency of retrograde motile events in dynein, dynactin, LIS1, and NDEL1 in 21–23 DPI neurons (dynein: 162 tracks, 21 videos, N = 6; dynactin: 68 tracks, 10 videos, N = 4; LIS1: 38 tracks, 14 videos, N = 6; NDEL1: 24 tracks, 10 videos, N = 3). Dynein versus NDEL1: **P = 0.0088, dynactin versus NDEL1: *P = 0.026, dynein versus dynactin: P = 0.81, LIS1 versus dynactin: P = 0.15, LIS1 versus dynein: P = 0.06, LIS1 versus NDEL1: P = 0.28, Kruskal–Wallis test, Dunn post hoc test. (C) Graph showing the speed of retrograde dynein, dynactin, LIS1, and NDEL1 particles in 21–23 DPI neurons. Dynein versus dynactin: P = 0.22, NDEL1 versus dynactin: P = 0.25, LIS1 versus dynactin: P = 0.07, dynein versus NDEL1: P = 0.90, dynein versus LIS1: P = 0.50, NDEL1 versus LIS1: P = 0.68. Boxplots shows median, first, and third quartiles. Upper/lower whiskers extend to 1.5× the interquartile range.

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