Figure 2.
Visualizing dynein in iNeurons. (A) Image of 21–23 DPI Halo-DYNC1H1 iNeuron axons stained with 1 nM JFX 554 and treated with 0.5 µM NEM. Teal and light green insets display spots before bleaching. (B) The bleaching trace from teal inset in A. The spot displays two bleaching steps representing the presence of one dynein molecule. (C) The bleaching trace from light green inset in A. The spot displays six bleaching steps representing the presence of three dynein molecules. (D) The number of bleaching steps from dynein (Halo-DYNC1H1) spots (389 spots, 14 videos, N = 3). (E) The number of bleaching steps from dynactin (Halo-ACTR10) spots (297 spots, 10 videos, N = 3). (F) Graph shows the analysis of step size intensity from dynein and dynactin spots during bleaching (dynein: 912 steps, 14 videos, N = 3; dynactin: 623 steps, 10 videos, N = 3). Boxplot shows median, first, and third quartiles. Upper/lower whiskers extend to 1.5× the interquartile range.

Visualizing dynein in iNeurons. (A) Image of 21–23 DPI Halo-DYNC1H1 iNeuron axons stained with 1 nM JFX 554 and treated with 0.5 µM NEM. Teal and light green insets display spots before bleaching. (B) The bleaching trace from teal inset in A. The spot displays two bleaching steps representing the presence of one dynein molecule. (C) The bleaching trace from light green inset in A. The spot displays six bleaching steps representing the presence of three dynein molecules. (D) The number of bleaching steps from dynein (Halo-DYNC1H1) spots (389 spots, 14 videos, N = 3). (E) The number of bleaching steps from dynactin (Halo-ACTR10) spots (297 spots, 10 videos, N = 3). (F) Graph shows the analysis of step size intensity from dynein and dynactin spots during bleaching (dynein: 912 steps, 14 videos, N = 3; dynactin: 623 steps, 10 videos, N = 3). Boxplot shows median, first, and third quartiles. Upper/lower whiskers extend to 1.5× the interquartile range.

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