Figure 6.
Cellular growth parameters of trappii mutants under single versus additive stress conditions. Seedlings were grown in the light (orange), dark (black), or dark with −0.4 MPa water stress (darkW; blue). (A and B) Scanning electron micrographs (SEM) of hypocotyls of (A) Col-0 wild type and (B) trs120-4, null trappii allele. The cell surface area was calculated as the product of cell width and length. trs120-4 mutants showed the opposite hypocotyl cell height and cell surface area adaptations to dark-to-darkW conditions (red asterisks in B) than the wild-type control. Representative SEM images of seedlings grown under the specified conditions are shown (scale bar = 40 µm). Outlines of representative cells are highlighted by white dashed lines. Shown are means ± SD. P values were computed with a two-tailed Student’s t test and are represented as follows: ***: P < 0.001; *****: P < 0.00001. (C and D) Confocal micrographs of mPS-PI stained (C) Col-0 wild type and (D) trs120-4 null mutant root tips under single or additive stress conditions; black arrowheads mark the junction between the meristematic and elongation zones for representative root tips. 10 days after incubation, the cell lengths were measured in single cortex cell files, starting at the cortex/endodermis initials. Cell lengths of consecutive cells were mapped as a function of cell number from the quiescent center (QC). The fitted lines were generated with Local Polynomial Regression Fitting with the “loess” method in R; gray shading designates the 95% confidence interval. Col-0 seedlings grown in the dark with and without water stress show steep slopes (green arrows). In the trappii mutant, the curves exhibit minimal to no difference between the different screen conditions (magenta arrows). Scale bar is 50 µm. The sample size (n) is given in each panel as the number of cells/number of seedlings that were analyzed (A–D). Related to Fig. S10.

Cellular growth parameters of trappii mutants under single versus additive stress conditions. Seedlings were grown in the light (orange), dark (black), or dark with −0.4 MPa water stress (darkW; blue). (A and B) Scanning electron micrographs (SEM) of hypocotyls of (A) Col-0 wild type and (B) trs120-4, null trappii allele. The cell surface area was calculated as the product of cell width and length. trs120-4 mutants showed the opposite hypocotyl cell height and cell surface area adaptations to dark-to-darkW conditions (red asterisks in B) than the wild-type control. Representative SEM images of seedlings grown under the specified conditions are shown (scale bar = 40 µm). Outlines of representative cells are highlighted by white dashed lines. Shown are means ± SD. P values were computed with a two-tailed Student’s t test and are represented as follows: ***: P < 0.001; *****: P < 0.00001. (C and D) Confocal micrographs of mPS-PI stained (C) Col-0 wild type and (D) trs120-4 null mutant root tips under single or additive stress conditions; black arrowheads mark the junction between the meristematic and elongation zones for representative root tips. 10 days after incubation, the cell lengths were measured in single cortex cell files, starting at the cortex/endodermis initials. Cell lengths of consecutive cells were mapped as a function of cell number from the quiescent center (QC). The fitted lines were generated with Local Polynomial Regression Fitting with the “loess” method in R; gray shading designates the 95% confidence interval. Col-0 seedlings grown in the dark with and without water stress show steep slopes (green arrows). In the trappii mutant, the curves exhibit minimal to no difference between the different screen conditions (magenta arrows). Scale bar is 50 µm. The sample size (n) is given in each panel as the number of cells/number of seedlings that were analyzed (A–D). Related to Fig. S10.

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