Figure S9.
Light responses in bin2-3bil1bil2, trappii, and echidna mutants. Seedlings were germinated on ½ MS in the light or dark. (A) Col-0 (wild type). (B) BR signaling mutant bin2-3bil1bil2 triple knockout (from Kalbfuß et al., 2022). (C)club-2, a null trappii allele. (D)trs120-4, a null trappii allele. (E) Null echidna allele, impaired in TGN structure and function. At least three experiments were performed for each line, and a representative one is shown here on the basis of RQ and P values. (F) Representative images of light-grown seedlings of Col-0 (wild type) and mutants shown in A–E. Dotted lines mark the hypocotyl-root junction, whereas arrows point to the end of the root. For corresponding dark-grown seedlings see Fig. 5 F. Scale bar is 1 cm. (G–I) Response quotients (RQ, left) and volcano plots (right) of (G) the hypocotyl, (H) the root, and (I) the hypocotyl/root ratio responses to light versus dark. RQs are normalized to the wild-type quotient; a value of 1 (vertical red line) indicates that the response to a shift from light to dark is identical to that of the respective wild-type ecotype. Each replicate is represented by a dot. Volcano plots show the mean RQ depicted on the X axis and the P value of the response on the Y axis (negative log scale; a median of all replicates was used). trappii mutants trs120-4 and club-2, the TGN mutant echidna and the higher order light perception null mutant phyAphyBcry1cry2 show a severely attenuated etiolation response (mean RQhypocotyl and mean RQratio >> 1.2 as well as RQroot << 0.8). Note that due to the opposite adaptations of the hypocotyl and root from light-to-dark conditions (increase in hypocotyl, decrease in root length and therefore increased hypocotyl/root ratio), the respective thresholds for attenuated responses are opposite (Fig. S9, G and I, compared with Fig. S9 H). The number (n) of seedlings measured per condition is in gray below the mean ± SD bar graphs. P values were computed with a two-tailed Student’s t test (*: P < 0.05; **: P < 0.01; ***: P < 0.001; ****: P < 0.0001; *****: P < 0.00001). Ecotypes are described in Table S2. All data points for A–E are shown in Fig. S7. Related to Fig. 5.

Light responses in bin2-3bil1bil2, trappii, and echidna mutants. Seedlings were germinated on ½ MS in the light or dark. (A) Col-0 (wild type). (B) BR signaling mutant bin2-3bil1bil2 triple knockout (from Kalbfuß et al., 2022). (C)club-2, a null trappii allele. (D)trs120-4, a null trappii allele. (E) Null echidna allele, impaired in TGN structure and function. At least three experiments were performed for each line, and a representative one is shown here on the basis of RQ and P values. (F) Representative images of light-grown seedlings of Col-0 (wild type) and mutants shown in A–E. Dotted lines mark the hypocotyl-root junction, whereas arrows point to the end of the root. For corresponding dark-grown seedlings see Fig. 5 F. Scale bar is 1 cm. (G–I) Response quotients (RQ, left) and volcano plots (right) of (G) the hypocotyl, (H) the root, and (I) the hypocotyl/root ratio responses to light versus dark. RQs are normalized to the wild-type quotient; a value of 1 (vertical red line) indicates that the response to a shift from light to dark is identical to that of the respective wild-type ecotype. Each replicate is represented by a dot. Volcano plots show the mean RQ depicted on the X axis and the P value of the response on the Y axis (negative log scale; a median of all replicates was used). trappii mutants trs120-4 and club-2, the TGN mutant echidna and the higher order light perception null mutant phyAphyBcry1cry2 show a severely attenuated etiolation response (mean RQhypocotyl and mean RQratio >> 1.2 as well as RQroot << 0.8). Note that due to the opposite adaptations of the hypocotyl and root from light-to-dark conditions (increase in hypocotyl, decrease in root length and therefore increased hypocotyl/root ratio), the respective thresholds for attenuated responses are opposite (Fig. S9, G and I, compared with Fig. S9 H). The number (n) of seedlings measured per condition is in gray below the mean ± SD bar graphs. P values were computed with a two-tailed Student’s t test (*: P < 0.05; **: P < 0.01; ***: P < 0.001; ****: P < 0.0001; *****: P < 0.00001). Ecotypes are described in Table S2. All data points for A–E are shown in Fig. S7. Related to Fig. 5.

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