![Characterization of claudin-1 and JAM-A mutants. (A–C) Localization of claudin-1 and its mutants in L cells. Claudin-1 and its mutants were ectopically expressed in L cells, which lack endogenous cell–cell adhesion activity. Claudin-1 full-length (FL; A) and claudin-1 [ΔYV] (C) were localized at cell–cell contacts (arrows), while claudin-1[F147A] (B) was not concentrated at cell–cell contacts. (D–F) Localization of JAM-A and its mutants in L cells. JAM-A full-length (D) and JAM-A[ΔLV] (F) were localized at cell–cell contacts (arrows), while JAM-A[ΔDL1] (E) was not localized at cell–cell contacts. (G–I) Double staining of claudin-1[FL] and ZO-1 in L cells. Claudin-1[FL] was able to recruit ZO-1 to cell–cell contacts (arrows). (J–L) Double staining of claudin-1[ΔYV] and ZO-1. Claudin-1[ΔYV] did not recruit ZO-1 to cell–cell contacts (arrowheads). (M–O) Double staining of JAM-A[FL] and ZO-1 in L cells. JAM-A[FL] was able to recruit ZO-1 to cell–cell contacts (arrows). (P–R) Double staining of JAM-A[ΔLV] and ZO-1. JAM-A[ΔLV] did not recruit ZO-1 to cell–cell contacts (arrowheads). Scale bars: 10 μm.](https://cdn.rupress.org/rup/content_public/journal/jcb/223/5/10.1083_jcb.202307104/2/jcb_202307104_figs4.png?Expires=1771177139&Signature=bw11IzUQ7DQXnwpzC1IDJrAf4Ljf1yveJbXLWZOTUcC4YA7zV6y3PIJu4qo8y2FQN7eCKLEO~akWgCcrdKM72tJBDnkFrU1IK2H3ZoPXmoKWJYrmP5oEjWCG3~-MVXct8XV2K-pRlhlJJJqkC9VD7Qc79vXXWaN2AMf3ji9GnpOtYVxLBu3advWqLuPtMfQos4hSAJYsq5CZpxESIAeo5320ESYTPF7r5pJMF64rKYn33EaNNSe~Pp17tyfX21erzzHwHGqHTSOxk1FPCcDr~41aCLhSj3dm3SYRqKQMPidrtJ~l5twWL4m1z7r0BWoqZyUu5BRcX1YNfIDUfVG-Fg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Characterization of claudin-1 and JAM-A mutants. (A–C) Localization of claudin-1 and its mutants in L cells. Claudin-1 and its mutants were ectopically expressed in L cells, which lack endogenous cell–cell adhesion activity. Claudin-1 full-length (FL; A) and claudin-1 [ΔYV] (C) were localized at cell–cell contacts (arrows), while claudin-1[F147A] (B) was not concentrated at cell–cell contacts. (D–F) Localization of JAM-A and its mutants in L cells. JAM-A full-length (D) and JAM-A[ΔLV] (F) were localized at cell–cell contacts (arrows), while JAM-A[ΔDL1] (E) was not localized at cell–cell contacts. (G–I) Double staining of claudin-1[FL] and ZO-1 in L cells. Claudin-1[FL] was able to recruit ZO-1 to cell–cell contacts (arrows). (J–L) Double staining of claudin-1[ΔYV] and ZO-1. Claudin-1[ΔYV] did not recruit ZO-1 to cell–cell contacts (arrowheads). (M–O) Double staining of JAM-A[FL] and ZO-1 in L cells. JAM-A[FL] was able to recruit ZO-1 to cell–cell contacts (arrows). (P–R) Double staining of JAM-A[ΔLV] and ZO-1. JAM-A[ΔLV] did not recruit ZO-1 to cell–cell contacts (arrowheads). Scale bars: 10 μm.
