Figure 6.
Actin cytoskeleton disorganization accompanies junction breakage in claudin/JAM-A KO cells. (A) Snapshots from a two-color time-lapse movie of claudin/JAM-A KO cells expressing ZO-1-GFP (green) and LifeAct-mCherry (magenta) showing a junction breakage event. The actin bundles were loosened and became detached from the cell junctions during the junction breakage (arrows). (B) Quantification of the junctional fluorescence intensities of ZO-1-GFP (green) and LifeAct-mCherry (magenta) during the junction breakage. The fluorescence intensities of ZO-1-GFP (green) and LifeAct-mCherry (magenta) were simultaneously reduced. The arrow indicates the start of the junction breakage event. (C) Snapshots from a two-color time-lapse movie of claudin/JAM-A KO cells expressing ZO-1-GFP (green) and LifeAct-mCherry (magenta) showing the junction recovery process after junction breakage. The boxed regions are enlarged in the right panels. The reassembly of the cell junction-associated actin bundles preceded the recovery of ZO-1-GFP junction accumulation (arrows). (D) Quantification of the junctional fluorescence intensities of ZO-1-GFP (green) and LifeAct-mCherry (magenta) during the junction recovery. The fluorescence intensity of LifeAct-mCherry (magenta) recovered before the fluorescence intensity of ZO-1-GFP (green) at the junction breakage site (arrows). Time scales: min:s. Scale bars: 10 μm.

Actin cytoskeleton disorganization accompanies junction breakage in claudin/JAM-A KO cells. (A) Snapshots from a two-color time-lapse movie of claudin/JAM-A KO cells expressing ZO-1-GFP (green) and LifeAct-mCherry (magenta) showing a junction breakage event. The actin bundles were loosened and became detached from the cell junctions during the junction breakage (arrows). (B) Quantification of the junctional fluorescence intensities of ZO-1-GFP (green) and LifeAct-mCherry (magenta) during the junction breakage. The fluorescence intensities of ZO-1-GFP (green) and LifeAct-mCherry (magenta) were simultaneously reduced. The arrow indicates the start of the junction breakage event. (C) Snapshots from a two-color time-lapse movie of claudin/JAM-A KO cells expressing ZO-1-GFP (green) and LifeAct-mCherry (magenta) showing the junction recovery process after junction breakage. The boxed regions are enlarged in the right panels. The reassembly of the cell junction-associated actin bundles preceded the recovery of ZO-1-GFP junction accumulation (arrows). (D) Quantification of the junctional fluorescence intensities of ZO-1-GFP (green) and LifeAct-mCherry (magenta) during the junction recovery. The fluorescence intensity of LifeAct-mCherry (magenta) recovered before the fluorescence intensity of ZO-1-GFP (green) at the junction breakage site (arrows). Time scales: min:s. Scale bars: 10 μm.

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