Figure 2.
Mechanical stress triggers junction breakage in claudin/JAM-A KO cells. (A) Snapshots from a movie of MDCK II cells expressing ZO-1-GFP. ZO-1-GFP maintained its continuity while the cells underwent stretching. (B) Snapshots from a movie of MDCK II cells expressing ZO-1-GFP, wherein a cell underwent cytokinesis. The junction continuity was maintained when cells underwent cytokinesis. (C) Snapshots from a movie of claudin/JAM-A KO cells expressing ZO-1-GFP. ZO-1-GFP became discontinuous or fragmented when a cell underwent acute stretching (arrows). The junctions were subsequently reassembled within 6 h. (D) Snapshots from a video of claudin/JAM-A KO cells expressing ZO-1-GFP. When a cell underwent cytokinesis, the junctions of the neighboring cells became discontinuous or fragmented (arrowheads). The junctions were reassembled within 2 h. (E) Quantification of the cell circumference (black) and ZO-1 intensity (green) during the cell stretching event in MDCK II cells shown in A. The ZO-1 intensity did not change significantly during the cell stretching. (F) Quantification of the cell circumference (black) and ZO-1 intensity (green) during the cell stretching event in claudin/JAM-A KO cells shown in C. The ZO-1 fluorescence intensity showed an acute reduction upon cell stretching. (G) Scatter plot of the cell stretching degrees and the ZO-1 intensity changes in multiple cell stretching events in MDCK II cells (circles) and claudin/JAM-A KO cells (blue triangles). Junction breakage events were highlighted by the filled blue triangles. In MDCK II cells, the ZO-1 intensity showed a mild reduction during cell stretching, which could have arisen from dilution of ZO-1 as the cell circumference increased. In claudin/JAM-A KO cells, the ZO-1 intensity did not change markedly when the cells underwent <25% stretching. However, a large reduction in ZO-1 intensity was observed when the cells were stretched by >25%, corresponding to the junction breakage events. The cells of interest are highlighted by pseudocolor and asterisks. Time scales: h:min. Scale bars: 10 μm.

Mechanical stress triggers junction breakage in claudin/JAM-A KO cells. (A) Snapshots from a movie of MDCK II cells expressing ZO-1-GFP. ZO-1-GFP maintained its continuity while the cells underwent stretching. (B) Snapshots from a movie of MDCK II cells expressing ZO-1-GFP, wherein a cell underwent cytokinesis. The junction continuity was maintained when cells underwent cytokinesis. (C) Snapshots from a movie of claudin/JAM-A KO cells expressing ZO-1-GFP. ZO-1-GFP became discontinuous or fragmented when a cell underwent acute stretching (arrows). The junctions were subsequently reassembled within 6 h. (D) Snapshots from a video of claudin/JAM-A KO cells expressing ZO-1-GFP. When a cell underwent cytokinesis, the junctions of the neighboring cells became discontinuous or fragmented (arrowheads). The junctions were reassembled within 2 h. (E) Quantification of the cell circumference (black) and ZO-1 intensity (green) during the cell stretching event in MDCK II cells shown in A. The ZO-1 intensity did not change significantly during the cell stretching. (F) Quantification of the cell circumference (black) and ZO-1 intensity (green) during the cell stretching event in claudin/JAM-A KO cells shown in C. The ZO-1 fluorescence intensity showed an acute reduction upon cell stretching. (G) Scatter plot of the cell stretching degrees and the ZO-1 intensity changes in multiple cell stretching events in MDCK II cells (circles) and claudin/JAM-A KO cells (blue triangles). Junction breakage events were highlighted by the filled blue triangles. In MDCK II cells, the ZO-1 intensity showed a mild reduction during cell stretching, which could have arisen from dilution of ZO-1 as the cell circumference increased. In claudin/JAM-A KO cells, the ZO-1 intensity did not change markedly when the cells underwent <25% stretching. However, a large reduction in ZO-1 intensity was observed when the cells were stretched by >25%, corresponding to the junction breakage events. The cells of interest are highlighted by pseudocolor and asterisks. Time scales: h:min. Scale bars: 10 μm.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal