RIC-7 is required for axonal mitochondria localization. (A) Representative images of mitochondria in AIY in WT, ric-7, and miro-1; ric-7 mutant animals. Arrows indicate mitochondria. Scale bar = 5 μm. (B) Representative images of mitochondria in DA9 in WT and ric-7 mutant animals. Arrows indicate mitochondria. Scale bar = 5 μm. (C) Diagram of DA9 neuron in the worm tail. Only the region shown on the micrographs in B is depicted. Microtubule polarity is indicated by + and −. (D–F) Quantification of mitochondria in AIY neurite, DA9 axon, and DA9 dendrite. Each data point represents one animal. Error bars represent SEM. Data are pooled from three independent experiments. Number of animals for AIY: WT = 15, ric-7 = 18, miro-1 = 19, miro-1; ric-7 = 11. Number of animals for DA9: WT = 12, ric-7 = 11. ****P < 0.0001 (one-way ANOVA with multiple comparisons for D, two-sided t test for E and F).