Figure 1.
Mitochondria anterograde trafficking requires kinesin-1/unc-116 and does not depend on miro or trak-1. (A) Representative images of mitochondria in AIY in WT and different mutant animals. Arrows indicate mitochondria in the axon. Scale bar = 5 μm. (B) Quantification of mitochondria in the neurite in WT and different mutant animals. Each data point represents one animal. Data are pooled from five independent experiments, total number of animals: WT = 13, kinesin-1 = 15, trak-1(KO) = 25, trak-1(lof) = 14, miro-1 = 26, and miro-1/2/3 = 17. Error bars represent SEM. ***P < 0.001, ****P < 0.0001, ns = not significant (one-way ANOVA with multiple comparisons). (C) Schematic diagram of the AIY neurons in the C. elegans head. The neurite is composed of three zones. Magenta dots in zone 2 and zone 3 indicate the clustering of presynaptic vesicles. Microtubule polarity is indicated by + and −.

Mitochondria anterograde trafficking requires kinesin-1/unc-116 and does not depend on miro or trak-1. (A) Representative images of mitochondria in AIY in WT and different mutant animals. Arrows indicate mitochondria in the axon. Scale bar = 5 μm. (B) Quantification of mitochondria in the neurite in WT and different mutant animals. Each data point represents one animal. Data are pooled from five independent experiments, total number of animals: WT = 13, kinesin-1 = 15, trak-1(KO) = 25, trak-1(lof) = 14, miro-1 = 26, and miro-1/2/3 = 17. Error bars represent SEM. ***P < 0.001, ****P < 0.0001, ns = not significant (one-way ANOVA with multiple comparisons). (C) Schematic diagram of the AIY neurons in the C. elegans head. The neurite is composed of three zones. Magenta dots in zone 2 and zone 3 indicate the clustering of presynaptic vesicles. Microtubule polarity is indicated by + and −.

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