Transition of Notch localization from raft-type membrane microdomain to non-raft domain. Cav-mRFP fluorescence intensity on each Notch-GFP spot was normalized by average intensity at the first five time points (from 0 to 40 s). (A and B) Notch-GFP remains within Caveolin-positive membrane microdomain over 10 min in Tsg101 KD cells (A), whereas the Cav-mRFP fluorescence declines in majority of Notch-GFP spots in Shrub KD cells (B).