Figure 7.
ESCRT-III dysfunction-induced lateral transition of Notch between membrane microdomains. (A–C) Time-lapse live cell imaging of Notch-EGFP (green), Cav1-mRFP (red), and SNAP-2xFYVE (blue) in Tsg101 KD (A), Shrub KD (B), and VPS4EQ overexpressing (C) S2R+ cells. (A) Individual Notch clusters remain within Cav1-positive domains on the limiting membrane (arrows and arrowheads). (B) In Shrub KD cells, Notch is initially found overlapping Cav1 positive (raft-type) membrane domain, but after 04′00″, Notch (arrows), and Cav1 (arrowheads) are separated into different microdomains of the endosome surface. (C) Although Notch clusters and the raft-like domains partially colocalize on the endosomal surface at time 00′00″, Notch (arrows) is gradually excluded from the Cav1 domains (arrowheads) and is sorted into distinguishable microdomains at 06′00″.

ESCRT-III dysfunction-induced lateral transition of Notch between membrane microdomains. (A–C) Time-lapse live cell imaging of Notch-EGFP (green), Cav1-mRFP (red), and SNAP-2xFYVE (blue) in Tsg101 KD (A), Shrub KD (B), and VPS4EQ overexpressing (C) S2R+ cells. (A) Individual Notch clusters remain within Cav1-positive domains on the limiting membrane (arrows and arrowheads). (B) In Shrub KD cells, Notch is initially found overlapping Cav1 positive (raft-type) membrane domain, but after 04′00″, Notch (arrows), and Cav1 (arrowheads) are separated into different microdomains of the endosome surface. (C) Although Notch clusters and the raft-like domains partially colocalize on the endosomal surface at time 00′00″, Notch (arrows) is gradually excluded from the Cav1 domains (arrowheads) and is sorted into distinguishable microdomains at 06′00″.

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