GPI-GFP is more likely to be localized between actin rings in progenitor-derived neuronal cells. (a) dSTORM reconstruction of actin in progenitor-derived neuronal cells stained with phalloidin. (b) Correlative SPT of GPI-GFP tagged with QDs and dSTORM of actin experiments in progenitor-derived neuronal cells. Top: GPI-GFP was tracked using QDs (5,000 frames, 200 Hz). Middle: Cells were subsequently fixed and stained for actin using phalloidin. Bottom: Overlay of SPT and STORM reconstructions. GPI-GFP is more likely to be localized between actin rings. (c) Autocorrelation function (ACF) along the dotted line in (b) reveals periodicity ∼200 nm of actin rings and GPI-GFP domains. Crosscorrelation function (XCF) of GPI-GFP domains and actin rings reveals that GPI-GFP is more likely to be localized between actin rings. Scale bars are 500 nm.