Figure 4.
Membrane domains can be detected for different membrane molecule species and in several cell types of the neuronal lineage. (a) Schematic representation of molecule species that were investigated: The inner leaflet peripheral membrane protein SRC-Halo, the transmembrane protein YFP-CB1, and the outer leaflet peripheral membrane protein GPI-GFP. (b) Top: Reconstruction of SPT experiment (20,000 frames, 100 Hz) of SRC-Halo tagged JF635; bottom: autocorrelation function (ACF) along the dotted line along the neuronal process shown on top reveals periodicity of SRC-Halo domains at ∼200 nm. (c) Top: Reconstruction of SPT experiment (20,000 frames, 200 Hz) of YFP-CB-1 tagged with QDs in progenitor-derived neuronal cells. Bottom: Autocorrelation function (ACF) along the dotted line along the neuronal process shown on top reveals the periodicity of YFP-CB1 domains at ∼200 nm. (d) Confocal images of progenitor-derived neuronal cells stained for their lineage marker: (left) merge, (middle left) neurons stained for TUJ, (middle right) astrocytes stained for GFAP, (right) oligodendrocytes stained for MBP. (e) Top: Reconstruction of SPT experiment (20,000 frames, 200 Hz) of GPI-GFP tagged with QDs in progenitor-derived neurons. Inset shows microscopy image of tracked cell stained for TUJ. Scale bar of inset is 10 µm. Bottom: Autocorrelation function (ACF) along the dotted line along the neuronal process shown on top reveals periodicity of GPI-GFP domains at ∼200 nm. (f) Like (e) but for an astrocyte stained for GFAP. (g) Like (e) but for an oligodendrocyte stained for MBP. If not otherwise indicated, scale bars are 500 nm.

Membrane domains can be detected for different membrane molecule species and in several cell types of the neuronal lineage. (a) Schematic representation of molecule species that were investigated: The inner leaflet peripheral membrane protein SRC-Halo, the transmembrane protein YFP-CB1, and the outer leaflet peripheral membrane protein GPI-GFP. (b) Top: Reconstruction of SPT experiment (20,000 frames, 100 Hz) of SRC-Halo tagged JF635; bottom: autocorrelation function (ACF) along the dotted line along the neuronal process shown on top reveals periodicity of SRC-Halo domains at ∼200 nm. (c) Top: Reconstruction of SPT experiment (20,000 frames, 200 Hz) of YFP-CB-1 tagged with QDs in progenitor-derived neuronal cells. Bottom: Autocorrelation function (ACF) along the dotted line along the neuronal process shown on top reveals the periodicity of YFP-CB1 domains at ∼200 nm. (d) Confocal images of progenitor-derived neuronal cells stained for their lineage marker: (left) merge, (middle left) neurons stained for TUJ, (middle right) astrocytes stained for GFAP, (right) oligodendrocytes stained for MBP. (e) Top: Reconstruction of SPT experiment (20,000 frames, 200 Hz) of GPI-GFP tagged with QDs in progenitor-derived neurons. Inset shows microscopy image of tracked cell stained for TUJ. Scale bar of inset is 10 µm. Bottom: Autocorrelation function (ACF) along the dotted line along the neuronal process shown on top reveals periodicity of GPI-GFP domains at ∼200 nm. (f) Like (e) but for an astrocyte stained for GFAP. (g) Like (e) but for an oligodendrocyte stained for MBP. If not otherwise indicated, scale bars are 500 nm.

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