Membrane domains can be detected in progenitor-derived neuronal cells. (a) Reconstruction of SPT experiment (20,000 frames, 200 Hz) of GPI-GFP tagged with QDs in progenitor-derived neuronal cells. (b) Zoom-in of box shown in (a) showing GPI-GFP forming membrane domains. (c) Autocorrelation function (ACF) along the dotted line along the neuronal process shown in (b) reveals periodicity of GPI-GFP domains at ∼200 nm. (d) Like (a) but different neuronal cell. (e) Single particle tracks color-coded for time of GPI-GFP motion of box shown in (d) reveals GPI-GFP membrane domains. (f) Like (c) but for neuron shown in (d). Scale bars are 500 nm.