Progenitor-derived neuronal cells do not show AnkG accumulation required for targeting of large channel proteins to the AIS. (a) Confocal image of a DIV7 rat hippocampal neuron stained for AnkG. Image shows characteristic AnkG accumulation in the AIS (see arrow). (b) Same as (a) but for DIV7 progenitor-derived neuronal cells. No AnkG accumulation. (c) Same as (a) but for DIV14 progenitor-derived neuronal cells. Even at DIV14 no AnkG accumulation can be detected. Scale bar is 10 µm.