Figure 9.
Induction of FIT target gene expression is enhanced under blue light. Gene expression analysis of total transcript abundance of iron deficiency genes FIT, BHLH039, FRO2, and IRT1 on 6-d-old Arabidopsis seedlings grown under white light for 5 d under iron deficient and iron sufficient conditions and then exposed for 1 d to blue, red, far-red light or darkness, or in parallel as control to white light. (A and B)FIT and bHLH039 gene induction in response to low iron supply did not change after blue light exposure compared to the white light control. (C and D)FRO2 and IRT1 gene expression increased after blue light exposure in iron-deficient conditions compared to respective iron-sufficient conditions and white light control. (E)FIT gene expression did not change after red light exposure compared to the white light control. (F and G)BHLH039 and FRO2 gene expression decreased after red light exposure in iron deficient conditions compared to respective iron sufficient condition and white light control. (H)IRT1 gene expression did not change after red light exposure compared to the white light control. (I)FIT gene expression did not change after far-red light exposure compared to the white light control. (J–L)BHLH039, FRO2, and IRT1 gene expression decreased after far-red light exposure in iron-deficient conditions compared with white light deficient condition comparable with the respective iron sufficient condition and iron sufficient white light control. (M)FIT gene expression was higher in darkness under iron-deficient conditions compared with respective iron sufficient condition and white light control. (N–P)BHLH039, FRO2, and IRT1 gene expression decreased after being exposed to darkness in iron-deficient conditions compared to white light-deficient conditions comparable with the respective iron-sufficient condition and iron-sufficient white light control. Two experiments were conducted, and one representative result is shown. Bar diagrams represent the mean and standard deviation of three replicates with 12 seedlings and two technical replicates each (n = 3). Statistical analysis was performed with one-way ANOVA and Tukey post-hoc test. Different letters indicate statistically significant differences (P < 0.05).

Induction of FIT target gene expression is enhanced under blue light. Gene expression analysis of total transcript abundance of iron deficiency genes FIT, BHLH039, FRO2, and IRT1 on 6-d-old Arabidopsis seedlings grown under white light for 5 d under iron deficient and iron sufficient conditions and then exposed for 1 d to blue, red, far-red light or darkness, or in parallel as control to white light. (A and B)FIT and bHLH039 gene induction in response to low iron supply did not change after blue light exposure compared to the white light control. (C and D)FRO2 and IRT1 gene expression increased after blue light exposure in iron-deficient conditions compared to respective iron-sufficient conditions and white light control. (E)FIT gene expression did not change after red light exposure compared to the white light control. (F and G)BHLH039 and FRO2 gene expression decreased after red light exposure in iron deficient conditions compared to respective iron sufficient condition and white light control. (H)IRT1 gene expression did not change after red light exposure compared to the white light control. (I)FIT gene expression did not change after far-red light exposure compared to the white light control. (J–L)BHLH039, FRO2, and IRT1 gene expression decreased after far-red light exposure in iron-deficient conditions compared with white light deficient condition comparable with the respective iron sufficient condition and iron sufficient white light control. (M)FIT gene expression was higher in darkness under iron-deficient conditions compared with respective iron sufficient condition and white light control. (N–P)BHLH039, FRO2, and IRT1 gene expression decreased after being exposed to darkness in iron-deficient conditions compared to white light-deficient conditions comparable with the respective iron-sufficient condition and iron-sufficient white light control. Two experiments were conducted, and one representative result is shown. Bar diagrams represent the mean and standard deviation of three replicates with 12 seedlings and two technical replicates each (n = 3). Statistical analysis was performed with one-way ANOVA and Tukey post-hoc test. Different letters indicate statistically significant differences (P < 0.05).

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