Vps13 is not required for cargo ubiquitination and ESCRT recruitment. (A) Schematic of a rapamycin-dependent degradation system for Can1. (B) Can1–FKBP localization. Rapamycin-insensitive mutant cells (tor1-1, fpr1Δ) expressing Can1–GFP–2xFKBP (Can1–FKBP) and FRB–3xUb were treated with rapamycin. (C) Can1–FKBP processing after rapamycin treatments. Cell lysates were analyzed by immunoblotting using anti-GFP and anti-G6PDH antibodies. (D) Quantification of Can-FKBP sorting from C. (E) Vps4–GFP localization. (F) Quantification of endosomal Vps4–GFP localization from E. Scale bar: 1 µm. Source data are available for this figure: SourceData F3.