Figure 1.
Vps13 is required for the ESCRT pathway. (A) Schematic of Mup1 sorting. (B) Mup1–GFP localization after methionine stimulation. (C) Western blotting analysis of Mup1 sorting. Cell lysates were analyzed by immunoblotting using anti-GFP and anti-G6PDH antibodies. Vacuole delivery of Mup1–GFP yields protease-resistant GFP fragments (GFP’). (D) Quantification of Mup1–GFP processing from C. (E) GFP–CPS localization. (F) Quantification of GFP–CPS localization of each category from E. (G) Schematic of Mup1–pHluorin assay. (H) Mup1–pHluorin localization after methionine stimulation. (I) Quantification of Mup1–pHluorin fluorescence at endosomes from H. (J) Vps10–GFP localization in WT, vps35Δ (retromer), and vps13Δ cells. (K) Quantification of Vps10–GFP localization from J. Scale bar: 1 µm. Source data are available for this figure: SourceData F1.

Vps13 is required for the ESCRT pathway. (A) Schematic of Mup1 sorting. (B) Mup1–GFP localization after methionine stimulation. (C) Western blotting analysis of Mup1 sorting. Cell lysates were analyzed by immunoblotting using anti-GFP and anti-G6PDH antibodies. Vacuole delivery of Mup1–GFP yields protease-resistant GFP fragments (GFP’). (D) Quantification of Mup1–GFP processing from C. (E) GFP–CPS localization. (F) Quantification of GFP–CPS localization of each category from E. (G) Schematic of Mup1–pHluorin assay. (H) Mup1–pHluorin localization after methionine stimulation. (I) Quantification of Mup1–pHluorin fluorescence at endosomes from H. (J) Vps10–GFP localization in WT, vps35Δ (retromer), and vps13Δ cells. (K) Quantification of Vps10–GFP localization from J. Scale bar: 1 µm. Source data are available for this figure: SourceData F1.

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