Figure S1.
Endosomal sorting pathways in vps13Δ cells. (A) CPY missorting index in vps mutants. The graph represents the percentage of secreted CPY-Invertase as reported by Robison et al. (1988). (B) Schematic of vacuolar protein sorting. (C) Localization of Pep4–GFP. (D) Quantification of Pep4–GFP localization from C. (E) Western blotting analysis of Pep4 sorting. Cell lysates were analyzed by immunoblotting using anti-Pep4 and anti-Pgk1 antibodies. (F) Localization of GFP–ALP and Vph1–mCherry. (G) Quantification of Vph1–mCherry localization from F. (H) Quantification of Vps10–GFP fluorescence from Fig. 1 J. (I) Kex2–GFP localization in WT, vps35Δ (retromer), and vps13Δ cells. (J) Quantification of Kex2–GFP localization from I. Scale bar: 1 µm. Source data are available for this figure: SourceData FS1.

Endosomal sorting pathways in vps13Δ cells. (A) CPY missorting index in vps mutants. The graph represents the percentage of secreted CPY-Invertase as reported by Robison et al. (1988). (B) Schematic of vacuolar protein sorting. (C) Localization of Pep4–GFP. (D) Quantification of Pep4–GFP localization from C. (E) Western blotting analysis of Pep4 sorting. Cell lysates were analyzed by immunoblotting using anti-Pep4 and anti-Pgk1 antibodies. (F) Localization of GFP–ALP and Vph1–mCherry. (G) Quantification of Vph1–mCherry localization from F. (H) Quantification of Vps10–GFP fluorescence from Fig. 1 J. (I) Kex2–GFP localization in WT, vps35Δ (retromer), and vps13Δ cells. (J) Quantification of Kex2–GFP localization from I. Scale bar: 1 µm. Source data are available for this figure: SourceData FS1.

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