Migfilin promotes pancreatic cancer cell growth. (A) Analysis of migfilin mRNA level in human pancreatic cancer tissues (Tumor) and matched adjacent normal tissues (Normal) by GEPIA webserver (https:/gepia.cancer-pku.cn/). Data represent mean ± SE, *P < 0.05, unpaired two-tailed t test. n = 179 for Tumor, n = 171 for Normal. (B) Kaplan-Meier plot showing migfilin (FBLIM1) expression in relation to pancreatic cancer patients’ disease-free survival rate. (C) Depletion of migfilin in KP4 led to a significant decrease in cell viability, as measured by MTT assay at the indicated time points. Data represent mean ± SE, ***P < 0.001, one-way ANOVA. n = 4 independent experiments. (D) Depletion of migfilin in KP4 cells led to a significant decrease in anchorage-dependent colony-forming abilities. Representative images (left panel) and quantification analysis (right panel) were shown. Data represent mean ± SE, ***P < 0.001, n.s., no significance, one-way ANOVA. n = 3 independent experiments. (E) Overexpression of SNAP29-QM or full-length migfilin significantly increased cell viability, compared to overexpression of SNAP29-WT (wild-type) or vector, in migfilin knockdown KP4 cells, as measured by MTT assay at the indicated time points. ***P < 0.001, one-way ANOVA. n = 4 independent experiments. (F) Overexpression of SNAP29-QM or full-length migfilin led to a significant increase in anchorage-dependent colony-forming abilities, compared to overexpression of SNAP29-WT or vector, in migfilin knockdown KP4 cells. Representative images (left panel) and quantification analysis (right panel) were shown. Data represent mean ± SE, *P < 0.05, **P < 0.01, one-way ANOVA. n = 3 independent experiments.