Depletion of migfilin increases focal adhesion accumulation. (A) Representative images of immunofluorescence staining for paxillin in cells as specified in the figure. Scale bar: 10 µm. (B) Quantification analysis of focal adhesion number per cell was shown. At least 35 cells in each group were analyzed. *P < 0.05, ***P < 0.001. n = 3 independent experiments. (C) Quantification analysis of total focal adhesion size per cell was shown. At least 35 cells in each group were analyzed. Data represent mean ± SE, *P < 0.05, **P < 0.01, ***P < 0.001, one-way ANOVA. n = 3 independent experiments.