Figure 4.
Migfilin promotes autophagosome–lysosome fusion. (A) Representative images showed that depletion of migfilin reduced the colocalization of GFP-LC3 with lysosomes stained by lysotracker red (Lyso-Red) under starvation conditions (upper panel). Overexpression of migfilin increased the colocalization of GFP-LC3 with lysosomes stained by lysotracker red (Lyso-Red) under starvation conditions (lower panel). Scale bar, 10 µm (Magnify, 2 µm). Quantification analysis was shown in the right panel. Data represent mean ± SE, **P < 0.01, ***P < 0.001, one-way ANOVA. n = 3 independent experiments. At least 35 cells for each group were analyzed. (B) Representative images showed that depletion of migfilin reduced the colocalization of endogenous LC3 with lysosomes labeled by Lamp1 under starvation condition (upper panel). Overexpression of migfilin increased the co-localization of endogenous LC3 with lysosomes labeled by Lamp1 under starvation conditions (lower panel). Scale bar, 10 µm (Magnify, 1 µm). Quantification analysis was shown in the right panel. Data represent mean ± SE, ***P < 0.001, one-way ANOVA. n = 3 independent experiments. At least 35 cells for each group were analyzed. (C) Representative images showed that depletion of migfilin reduced the colocalization of endogenous LC3 with late endosomes labeled by mCherry-Rab7 under starvation conditions (upper panel). Overexpression of migfilin increased the co-localization of GFP-LC3 with late endosomes labeled by mCherry-Rab7 under starvation conditions (lower panel). Scale bar, 10 µm (Magnify, 2 µm). Quantification analysis was shown in the right panel. Data represent mean ± SE, ***P < 0.001, one-way ANOVA. n = 3 independent experiments. At least 20 cells for each group were analyzed.

Migfilin promotes autophagosome–lysosome fusion. (A) Representative images showed that depletion of migfilin reduced the colocalization of GFP-LC3 with lysosomes stained by lysotracker red (Lyso-Red) under starvation conditions (upper panel). Overexpression of migfilin increased the colocalization of GFP-LC3 with lysosomes stained by lysotracker red (Lyso-Red) under starvation conditions (lower panel). Scale bar, 10 µm (Magnify, 2 µm). Quantification analysis was shown in the right panel. Data represent mean ± SE, **P < 0.01, ***P < 0.001, one-way ANOVA. n = 3 independent experiments. At least 35 cells for each group were analyzed. (B) Representative images showed that depletion of migfilin reduced the colocalization of endogenous LC3 with lysosomes labeled by Lamp1 under starvation condition (upper panel). Overexpression of migfilin increased the co-localization of endogenous LC3 with lysosomes labeled by Lamp1 under starvation conditions (lower panel). Scale bar, 10 µm (Magnify, 1 µm). Quantification analysis was shown in the right panel. Data represent mean ± SE, ***P < 0.001, one-way ANOVA. n = 3 independent experiments. At least 35 cells for each group were analyzed. (C) Representative images showed that depletion of migfilin reduced the colocalization of endogenous LC3 with late endosomes labeled by mCherry-Rab7 under starvation conditions (upper panel). Overexpression of migfilin increased the co-localization of GFP-LC3 with late endosomes labeled by mCherry-Rab7 under starvation conditions (lower panel). Scale bar, 10 µm (Magnify, 2 µm). Quantification analysis was shown in the right panel. Data represent mean ± SE, ***P < 0.001, one-way ANOVA. n = 3 independent experiments. At least 20 cells for each group were analyzed.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal