Figure S1.
Conservations and ciliary localizations of Kif6 and Kif9. Related to Fig. 1. (A) Similarities of Kif6 (left) and Kif9 (right) orthologues, obtained using constraint-based multiple alignment tool (COBALT) in NCBI. Highly conserved positions are shown in red and less conserved positions in blue. Protein sequences of Kif6 orthologues used are mouse (NP_796026.2), human (NP_659464.3), chicken (XP_003640993.5), frog (NP_001106482.1), zebrafish (NP_001070899.1), lancelet (XP_035685117.1), ascidian (XP_002124970.1), sea urchin (XP_030854411.1), mussel (CAG2221785.1), flatworm (PAA61917.1), sea anemone (XP_032228212.2), Trichoplax (RDD42533.1), Tetrahymena (XP_001024804.2), Trypanosoma (XP_846346.1), and green algae (XP_042928646.1). Protein sequences of Kif9 orthologues used were: mouse (NP_001157041.1), human (NP_001400904.1), chicken (XP_040522129.1), frog (XP_012821147.1), zebrafish (XP_001922460.1), lancelet (XP_035663761.1), ascidian (XP_018668035.1), sea urchin (XP_030830023.1), mussel (CAG2242108.1), flatworm (PAA61899.1), sea anemone (XP_001636554.1), Trichoplax (RDD45058.1), Tetrahymena (XP_001022313.1), Trypanosoma (RHW71316.1), and green algae (P46870.1). (B) Sequence alignment of Kif6 and Kif9 motor domains, generated by the COBALT sequence alignment program using default parameters. Their GenBank accession numbers are NP_796026.2 (Kif6) and NP_001157041.1 (Kif9). The motor domains were identified using SMART. (C) Localizations of exogenous Kif6 and Kif9 in motile cilia. Cultured mEPCs were treated as indicated in Fig. 1 G to express Kif6-GFP or Kif9-GFP and imaged by 3D-SIM. Spef1 and acetylated tubulin (Ac-tub) served as markers for the CP and the axoneme, respectively. Typical cilia (arrowheads) were magnified by 200% to highlight details. Line scans were generated at the indicated positions by white lines in merged images.

Conservations and ciliary localizations of Kif6 and Kif9. Related to Fig. 1. (A) Similarities of Kif6 (left) and Kif9 (right) orthologues, obtained using constraint-based multiple alignment tool (COBALT) in NCBI. Highly conserved positions are shown in red and less conserved positions in blue. Protein sequences of Kif6 orthologues used are mouse (NP_796026.2), human (NP_659464.3), chicken (XP_003640993.5), frog (NP_001106482.1), zebrafish (NP_001070899.1), lancelet (XP_035685117.1), ascidian (XP_002124970.1), sea urchin (XP_030854411.1), mussel (CAG2221785.1), flatworm (PAA61917.1), sea anemone (XP_032228212.2), Trichoplax (RDD42533.1), Tetrahymena (XP_001024804.2), Trypanosoma (XP_846346.1), and green algae (XP_042928646.1). Protein sequences of Kif9 orthologues used were: mouse (NP_001157041.1), human (NP_001400904.1), chicken (XP_040522129.1), frog (XP_012821147.1), zebrafish (XP_001922460.1), lancelet (XP_035663761.1), ascidian (XP_018668035.1), sea urchin (XP_030830023.1), mussel (CAG2242108.1), flatworm (PAA61899.1), sea anemone (XP_001636554.1), Trichoplax (RDD45058.1), Tetrahymena (XP_001022313.1), Trypanosoma (RHW71316.1), and green algae (P46870.1). (B) Sequence alignment of Kif6 and Kif9 motor domains, generated by the COBALT sequence alignment program using default parameters. Their GenBank accession numbers are NP_796026.2 (Kif6) and NP_001157041.1 (Kif9). The motor domains were identified using SMART. (C) Localizations of exogenous Kif6 and Kif9 in motile cilia. Cultured mEPCs were treated as indicated in Fig. 1 G to express Kif6-GFP or Kif9-GFP and imaged by 3D-SIM. Spef1 and acetylated tubulin (Ac-tub) served as markers for the CP and the axoneme, respectively. Typical cilia (arrowheads) were magnified by 200% to highlight details. Line scans were generated at the indicated positions by white lines in merged images.

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