BLTPs possess a long hydrophobic cavity that can be filled with multiple lipids. (A–F) (A–E) Spatial density maps and (F) lipid tail solvation (per lipid) using the iterative lipid addition process for BLTPs: (A) Atg2, (B) Vps13, (C) Csf1, and mutants of (D) Atg2 and (E) Vps13. Proteins are shown in purple; averaged lipid tail densities are in green. The mutated residues are displayed in blue (arginine, lysine) or red (glutamate, aspartate).