Lipid entry pathways. (A) Entry pathway of lipids into the LTD. The percentage values beside the arrows indicate the fraction of replicas in which the pathway was observed. Proteins are represented in cartoon representation and the center of mass of the lipid along the trajectory, neglecting the frames in which the lipid is in bulk solvent, represented as spheres. Each color of the sphere corresponds to a different replica with lipid binding. (B and C) Mutation of residues into disulfide bridges along the lipid-entry path in NF1 and PITP abolishes lipid entry into the binding pocket as indicated by the lipid solvation plots. The use of a different force constant for the elastic network of StARD11 is indicated by a *.