Figure 6.
The MDC lumen contains cytosolic material. (A) Super-resolution confocal fluorescence microscopy images of Rap-induced MDC formation in yeast expressing yEGFP and Tom70-mCherry. MDCs are indicated by white arrows. Scale bar = 1 µm. The yellow line marks the position of the line-scan fluorescence intensity profile shown to the right. The left and right y axes correspond to yEGFP and Tom70-mCherry fluorescence intensity, respectively. *Panels showing an increased pixel intensity to observe Tom70-mCherry–marked mitochondrial tubules, indicated with a yellow arrow. (B) Super-resolution confocal fluorescence microscopy images of swollen mitochondria (yellow arrows) from yeast expressing yEGFP, Tom70-mCherry, Mdm12-AID-6xFLAG, and OsTir1. Images were taken 3 h after treatment with 1 mM auxin, which acutely swelled mitochondria through auxin-induced degradation of Mdm12-AID-6xFLAG. Scale bar = 1 µm. The yellow line marks the position of the line-scan fluorescence intensity profile shown to the right. Left and right y axes correspond to yEGFP and Tom70-mCherry fluorescence intensity, respectively. (C and D) Representative super-resolution confocal fluorescence microscopy images of yeast expressing yEGFP-IAA7 and Tom70-mCherry. After a two-hour treatment with 200 nM Rap, cells were subsequently treated with 70% ethanol (C; Rap + Veh) or 1 mM auxin (D; Rap + Auxin). Yellow arrows mark mitochondria while white arrows mark MDCs. Scale bar = 1 µm. The yellow line denotes the position of the line-scan fluorescence intensity profile shown to the right. The left and right y axes correspond to GFP and Tom70-mCherry fluorescence intensity, respectively. The blue percentages next to the panels shown in D indicate the frequency those results were observed from n = 106 MDCs from four experiments.

The MDC lumen contains cytosolic material. (A) Super-resolution confocal fluorescence microscopy images of Rap-induced MDC formation in yeast expressing yEGFP and Tom70-mCherry. MDCs are indicated by white arrows. Scale bar = 1 µm. The yellow line marks the position of the line-scan fluorescence intensity profile shown to the right. The left and right y axes correspond to yEGFP and Tom70-mCherry fluorescence intensity, respectively. *Panels showing an increased pixel intensity to observe Tom70-mCherry–marked mitochondrial tubules, indicated with a yellow arrow. (B) Super-resolution confocal fluorescence microscopy images of swollen mitochondria (yellow arrows) from yeast expressing yEGFP, Tom70-mCherry, Mdm12-AID-6xFLAG, and OsTir1. Images were taken 3 h after treatment with 1 mM auxin, which acutely swelled mitochondria through auxin-induced degradation of Mdm12-AID-6xFLAG. Scale bar = 1 µm. The yellow line marks the position of the line-scan fluorescence intensity profile shown to the right. Left and right y axes correspond to yEGFP and Tom70-mCherry fluorescence intensity, respectively. (C and D) Representative super-resolution confocal fluorescence microscopy images of yeast expressing yEGFP-IAA7 and Tom70-mCherry. After a two-hour treatment with 200 nM Rap, cells were subsequently treated with 70% ethanol (C; Rap + Veh) or 1 mM auxin (D; Rap + Auxin). Yellow arrows mark mitochondria while white arrows mark MDCs. Scale bar = 1 µm. The yellow line denotes the position of the line-scan fluorescence intensity profile shown to the right. The left and right y axes correspond to GFP and Tom70-mCherry fluorescence intensity, respectively. The blue percentages next to the panels shown in D indicate the frequency those results were observed from n = 106 MDCs from four experiments.

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