The MDC lumen contains cytosolic material. (A) Super-resolution confocal fluorescence microscopy images of yeast cells expressing Tom70-GFP-IAA7 and Tom70-mCherry. Images were taken 2 h after treatment with 1 mM auxin, which was added after a 2-h pretreatment with Rap (Rap + Aux). MDCs are indicated by white arrows. Scale bar = 1 µm. (B) Super-resolution confocal fluorescence microscopy images of MDC formation in yeast expressing yEGFP and Tom70-mCherry. MDCs were induced with either ConcA, CHX, or overexpression of the OMM protein Scm4 (GPDpr::SCM4). MDCs are indicated by white arrows. Scale Bar = 1 µm. The yellow line marks the position of the line-scan fluorescence intensity profile shown to the right. The left and right y axes correspond to yEGFP and Tom70-mCherry fluorescence intensity, respectively. (C) Representative super-resolution confocal fluorescence microscopy images of yeast expressing yEGFP-IAA7 and Tom70-mCherry. After a 2-h treatment with ConcA or CHX, cells were subsequently treated with 1 mM auxin (ConcA + Aux and CHX + Aux). Cells overexpressing the OMM protein Scm4 (GPDpr::SCM4) were treated with 1 mM auxin (GPDpr::SCM4 + Aux). White arrows mark MDCs. Scale bar = 1 µm. The yellow line denotes the position of the line-scan fluorescence intensity profile shown to the right. The left and right y axes correspond to GFP and Tom70-mCherry fluorescence intensity, respectively.