Figure S1.
ConcA-treated yeast also produce mitochondrial-derived multilamellar structures. (A) Super-resolution confocal fluorescence microscopy images of haploid yeast expressing Tom70-yEGFP and Tim50-mCherry treated with either DMSO or 500 nM ConcA. MDCs are indicated by white arrows. Scale bar = 1 µm. The yellow line marks the position of the line-scan fluorescence intensity profile shown to the right. The left and right y axes correspond to Tom70-yEGFP and Tim50-mCherry fluorescence intensity, respectively. Bracket marks MDC. (B) Quantification of MDC formation in DMSO or ConcA-treated yeast. Error bars show mean ± SE of three replicates, n ≥ 100 cells per replicate. (C) Scatter plot showing the diameter of ConcA-induced MDCs. The black line indicates the mean (0.36 µm) of n = 49 MDCs. (D) Thin-section TEM analysis of 80-nm cell sections from the same yeast strain analyzed above treated with 500 nM ConcA. Sections were stained with monoclonal antibodies targeting GFP and secondary antibodies conjugated to 10-nm gold particles. White arrow: multilamellar structures, white arrowheads point to gold particles, M: mitochondria. Scale bar = 200 nm. (E) Quantification of the total anti-GFP immunogold particles that labeled the indicated cell structures from an analysis of >100 cell sections from yeast expressing Tom70-yEGFP that were treated with either DMSO (vehicle) or 500 nM ConcA.

ConcA-treated yeast also produce mitochondrial-derived multilamellar structures. (A) Super-resolution confocal fluorescence microscopy images of haploid yeast expressing Tom70-yEGFP and Tim50-mCherry treated with either DMSO or 500 nM ConcA. MDCs are indicated by white arrows. Scale bar = 1 µm. The yellow line marks the position of the line-scan fluorescence intensity profile shown to the right. The left and right y axes correspond to Tom70-yEGFP and Tim50-mCherry fluorescence intensity, respectively. Bracket marks MDC. (B) Quantification of MDC formation in DMSO or ConcA-treated yeast. Error bars show mean ± SE of three replicates, n ≥ 100 cells per replicate. (C) Scatter plot showing the diameter of ConcA-induced MDCs. The black line indicates the mean (0.36 µm) of n = 49 MDCs. (D) Thin-section TEM analysis of 80-nm cell sections from the same yeast strain analyzed above treated with 500 nM ConcA. Sections were stained with monoclonal antibodies targeting GFP and secondary antibodies conjugated to 10-nm gold particles. White arrow: multilamellar structures, white arrowheads point to gold particles, M: mitochondria. Scale bar = 200 nm. (E) Quantification of the total anti-GFP immunogold particles that labeled the indicated cell structures from an analysis of >100 cell sections from yeast expressing Tom70-yEGFP that were treated with either DMSO (vehicle) or 500 nM ConcA.

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