Figure S4.

Docked centrioles in adult GCs lack directed orientation. (A) Serial EM sections display the only cilium annotated in the adult IGL volumes. Internal vesicles are present and the axoneme is not present or not resolved. Scale bar is 200 nm. (B) To assess the polarity of centriole docking, we generated a vector from proximal to distal within each mother centriole. We compared the vectors and found no bias in the orientation of docked centrioles. (C) The type of structure immediately adjacent to the plasma membrane where each GC mother centriole docked was determined. The distributions for the annotated centrioles in each adult dataset are plotted on the left and EM images on the right provide examples of centrioles docked adjacent to the indicated structures.

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